Protein Purification MSDS

Materials Safety Data Sheets (MSDS) for all chemicals, solutions, and organisms involved in the Protein Purification Lab. For specific concentrations of final mixtures (buffers), please request the SOP for that solution from Heather Gastil (hgastil@ucsd.edu).

Documents

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Ampicillin MSDS Ampicillin MSDS

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Date added: 01/12/2012
Date modified: 01/12/2012
Filesize: 173.44 kB
Downloads: 461
This MSDS is for the Ampicillin Sodium salt, used in an ampicillin solution of 100mg/mL in this lab.  

E. coli MSDS E. coli MSDS

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Date added: 01/12/2012
Date modified: 01/12/2012
Filesize: 117.15 kB
Downloads: 458
This MSDS is for our E. coli strain: NEB Turbo cells. 

EDTA Disodium MSDS EDTA Disodium MSDS

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Date added: 01/12/2012
Date modified: 01/12/2012
Filesize: 209.52 kB
Downloads: 470
This MSDS is for EDTA (used at a concentration of 0.5M and pH 8 in this lab) as part of the TE Buffer and Elution Buffer. 

Elution Buffer MSDS Elution Buffer MSDS

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Date added: 01/12/2012
Date modified: 01/12/2012
Filesize: 304.32 kB
Downloads: 459
This MSDS is for Elution Buffer (EB), formed from 1M Imidazole solution, 1M Tris (pH 8), and 0.5M EDTA (pH 8). MSDS sheets for individual components can be found as separate files here as well. 

Imidazole MSDS Imidazole MSDS

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Date added: 01/12/2012
Date modified: 01/12/2012
Filesize: 144.41 kB
Downloads: 462
This MSDS is for Imidazole (used at 1M in this lab). 

LB Broth MSDS LB Broth MSDS

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Date added: 01/12/2012
Date modified: 01/12/2012
Filesize: 94.36 kB
Downloads: 438
This MSDS is for LB Broth, used at 40g/L in this lab. 

Lysozyme MSDS Lysozyme MSDS

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Date added: 01/12/2012
Date modified: 01/12/2012
Filesize: 153.88 kB
Downloads: 442
This MSDS is for Lysozyme (from chicken egg), using 0.025g of Lysozyme per tube in this lab. 

Nickel beads MSDS Nickel beads MSDS

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Date added: 01/12/2012
Date modified: 01/12/2012
Filesize: 88.72 kB
Downloads: 432
This MSDS is for Nickel beads: Invitrogen ProBond Resin (150 microliters per tube).

TE Buffer MSDS TE Buffer MSDS

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Date added: 01/12/2012
Date modified: 01/12/2012
Filesize: 110.61 kB
Downloads: 452
This MSDS is for TE Buffer, composed of 1M Tris (pH 8), 0.5M EDTA (pH 8), and water. 

Tris MSDS Tris MSDS

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Date added: 01/12/2012
Date modified: 01/12/2012
Filesize: 115.63 kB
Downloads: 456
This MSDS is for Tris base, which, when mixed with water, is used at 1M for this lab.